As the pcr reaction proceeds at each round of amplification sybr green dye binds to dsdna as it polymerizes resulting in an increase in the level of fluorescence at the end of each extension step. Amplitaq fast dna polymerase up a highly purified dna polymerase designed to allow instant hot start minimizing non specific product formation and enabling reactions to be set up at room temperature. Rt 2 sybr green fast mastermixes are available with rox fluorescein or without reference dyes.
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